[Zurück]

S. Helfert, C. Dworak, E. Sevcsik, H. Peterlik, M. Sauer, D. Ret, R. Liska:
"Synthesis and Characterization of Polymer Linker Systems for T-cell Investigations";
Poster: Workshop Additive manufacturing as a tool to create life in 3D, Brno; 26.07.2017 - 27.07.2017.

In the field of biomedical applications, the generation and design of polymer brushes became very attractive, due to their tunable architectural features and chemical characteristics.
Those adjustable properties offer the creation of particular biointerfaces and applications in nanotechnologies on a variety of substrates [1].
In this work, a strategy for a specific biointerface between T-cells and SiO2 substrates was prearranged by the utilization of Reversible Addition-Fragmentation chain Transfer (RAFT)
polymerization. RAFT polymerization is a controlled radical polymerization technique and a powerful tool in the generation of polymers with well-defined architectures [2] under gentle
conditions and absence of toxic catalysts. 20 kDa polymers of N-acrylolmorpholine (NAM) and N-(3-methoxypropyl) acrylamide (MPAA) were prepared via RAFT polymerization to
determine the reaction kinetics by usage of two different trithiocarbonate RAFT reagents and their morphology in aqueous environment was investigated to predict their arrangement in a
polymer brush system. Further studies were applied for end-group modifications via aminolysis and radical induced end-group modification of the appropriate polymers to prepare a later
coupling to biomolecules. For the generation of the proper polymer brushes in a "grafting from" mode, the RAFT reagents were modified with a trialkoxysilane group and coupled to flat
SiO2 surfaces.
The monomer conversion of the polymerization was monitored via 1H-NMR and the molecular weight distributions were determined via size exclusion chromatography (SEC). End-group
modifications were confirmed via UV/VIS spectroscopy and SEC. Morphology and shape was investigated via static light scattering (SLS) and small angle X-ray scattering (SAXS).
Contact angle, ellipsometry and X-ray photoelectron spectroscopy (XPS) were used for surface characterizations.

biomedical applications, the generation and design of polymer brushes, tunable architectural features and chemical characteristics, biointerfaces and applications in nanotechnologies on a variety of substrates