[Zurück]

M. Lunzer, D. Ossipov, P. Gruber, K. Hölzl, M. Markovic, R. Liska, A. Ovsianikov:
"Biocompatible Micropatterning of o-Nitrobenzyl Crosslinked Hydrogels by Sensitised Two-Photon Cleavage";
Poster: Workshop Additive manufacturing as a tool to create life in 3D, Brno; 26.07.2017 - 27.07.2017.

Hydrogels are useful biomaterials for various strategies in tissue engineering, regenerative medicine and cell delivery, as they permit to mimic the physicochemical
properties of the natural extracellular matrix (ECM). Since cells in vivo are surrounded by a complex and alterable 3D environment, dynamically tuneable
biomaterials are highly relevant for research on cell and tissue physiology [1]. Photoresponsive hydrogels allow for real-time control of the biomaterial´s chemical
and mechanical properties at locations of interest. Photodegradability of such biomaterials is commonly enabled by the integration of photolabile
o-nitrobenzyl (oNB) ester derivatives, which can be photocleaved either by UV light irradiation or by a two-photon process applying pulsed IR-laser light [2]. The
later mode of excitation is of particular interest for high resolution 3D-micropatterning in presence of cells. To increase the efficiency of this two-photon induced
process, we present a modular system permitting the sensitization of the oNB photoscission by addition of a small molecule two-photon chromophore.

Hydrogels, biomaterials for various strategies in tissue engineering,