[Zurück]

M. Rothbauer, N. Patel, G. Hajnalka, M. Siewetz, B. Huppertz, P. Ertl:
"A comparative study of five physiological key parameters between four different human trophoblast-derived cell lines";
Scientific Reports, 7 (2017).

The human placenta plays a crucial role as the interface between mother and fetus. It represents a
unique tissue that undergoes morphological as well as functional changes on the cellular and tissue
level throughout pregnancy. To better understand how the placenta works, a variety of techniques
has been developed to re-create this complex physiological barrier in vitro. However, due to the low
availability of freshly isolated primary cells, choriocarcinoma cell lines remain the usual suspects as in
vitro models for placental research. Here, we present a comparative study on the functional aspects
of the choriocarcinoma cell lines BeWo, JAR and Jeg-3, as well as the first trimester trophoblast cell
line ACH-3P as placental in vitro barrier models for endocrine and transport studies. Functional assays
including tight junction immunostaining, sodium fluorescein retardation, trans epithelial resistance,
glucose transport, hormone secretion as well as size-dependent polystyrene nanoparticle transport
were performed using the four cell types to evaluate key functional parameters of each cell line to act a
relevant in vitro placental barrier model.

placenta; cell lines; choriocarcinoma; barrier model; transport; nanoparticles; β-hCG; glucose, BeWo, JAR, Jeg-3, ACH-3P

http://dx.doi.org/10.1038/s41598-017-06364-z

http://publik.tuwien.ac.at/files/publik_261366.pdf