[Zurück]

D. Ret, G. Mihlaç, S. Gentilini, S. Knaus:
"Proton Mobility of Hyaluronan and Derivatives in Solution";
Vortrag: Danube Vltava Sava Polymer Meeting 2017, Wien; 05.09.2017 - 08.09.2017; in: "Danube Vltava Sava Polymer Meeting 2017", Book of Abstracts.com, (2017), ISBN: 978-3-9504017-6-9; S. OP-76.

Hyaluronic acid (HA) is a biomaterial used in medical and esthetic application such as
eye surgery, viscosupplementation therapy of osteoarthritis, dermal filling, and tissue
augmentation. To improve the chemical and physical properties in biological systems
hyaluronic acid is chemically modified. In establishment of the relationship between
different degrees of modification and mechanical properties as well as biocompatibility
the accurate determination of the degree of substitution (DS) is of great importance.
An effective and wide spread method for determining the DS is 1H-NMR spectroscopy.
This method gives accurate results only when the implied protons are mobile [1].
Hyaluronic acid proton mobility is affected by many parameters as: molecular weight,
concentration, ionic force, pH and temperature.
In the present work the determination of the DS was intensively studied and the
relationship between particle size, conformation in solution and proton mobility was
elucidated. It will be shown that accurate determination of the DS by 1H-NMR
spectroscopy for HA derivatives is possible (Figure 1) and that the same principle can
be used for other glycosaminoglycans.

Hyaluronic acid, medical and esthetic application, chemically modified, relationship between particle size, conformation in solution and proton mobility