[Zurück]

D. Ret, M. Missbach, S. Knaus:
"SEC with conventional calibration: Dos and Don´ts";
Poster: Danube Vltava Sava Polymer Meeting 2017, Wien; 05.09.2017 - 08.09.2017; in: "Danube Vltava Sava Polymer Meeting 2017", Book of Abstracts.com, (2017), ISBN: 978-3-9504017-6-9; S. PO-49.

Gel Permeation Chromatography (GPC), also known as Size Exclusion Chromatography (SEC) is a very common technique for polymer molecular weight determination. The
use of different detectors in addition to the standard RI detector, such as UV, viscosity and multi angle light scattering detectors provides access to absolute molar masses
(Mw) and offers additional information about composition, branching and structure of polymers. However, such an equipment is rather expensive, a lot of expertise is
required and eluent change is time consuming. Thus, very often SEC analysis is done only with conventional calibration (Figure 1). Independent on the system used for
analysis it is very important to consider factors such as sample concentration, which might be influenced by filtration, and conformation of standard and sample in
solution, which is strongly dependent on polymer/polymer and polymer/solvent interaction. In this poster some examples of Mw determination of water soluble polymers,
polyelectrolytes and proteins using the SEC equipment of Orange Analytic (Figure 2), a user-friendly, robust an cost-efficient instrument, are shown and dos and don´ts are
discussed.

Gel Permeation Chromatography (GPC), polymer molecular weight characterization, eluent composition, sample concentration and filtration methods,