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S. Baudis, E. Zerobin, M. Lunzer, M. Tromayer, M. Markovic, P. Gruber, J. Stampfl, A. Ovsianikov, R. Liska:
"Macromolecular Photochemistry: A Toolbox to Control the Micro-Environment of Cells";
Vortrag: 17. Österreichische Chemietage 2017, Salzburg; 25.09.2017 - 28.09.2017; in: "17. Österreichische Chemietage 2017", (2017), S. OP-29.

Multi-photon-processes are nonlinear optical processes taking place at high photon densities in the focus of femtosecond pulsed lasers hence can trigger photochemistry in confined space (< 1 µm) inside materials.[1] Here, a toolbox of biocompatible photochemistry is presented, which enables the control of the micro-environment of living cells. By employing two-photon-initiators (2PI) with either photo-crosslinking or photo-degrading macromers cell-photoencapsulation or photo-milling of channels for directed cell growth is enabled.
Hydrogel precursors based on natural (e.g., gelatin, hyaluronan, etc.) or biocompatible synthetic polymers (e.g., poly(vinyl alcohol)) are materials of choice. These were modified with ene functionalities (e.g., methacrylates, vinylesters, norbornenes) or thiols and coupled with (macromolecular) crosslinkers by photo-thiol-ene reactions to form hydrogel networks with arbitrary shape in high resolution. By introduction of photo-cleavable groups (e.g., o-nitrobenzylester) into the hydrogel networks the photo-degradation of formed crosslinks by the two-photon-process is enabled. On the whole a spatial-temporal control of the hydrogel properties in the presence of living cells is approached by combination of the presented material platforms with two-photon-technology. Therefore, this technology is an ideal tool to study the behavior of cells in different micro-environments.[2]

Multi-photon-processes, photochemistry, biocompatible photochemistry, spatial-temporal control of the hydrogel properties in the presence of living cells, tool to study the behavior of cells in different micro-environments

http://publik.tuwien.ac.at/files/publik_261534.pdf