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A. Balbekova, M. Bonta, S. Toeroek, J. Ofner, B. Doeme, A. Limbeck, B. Lendl:
"FTIR-spectroscopic and LA-ICP-MS imaging for combined hyperspectral image analysis of tumor models";
Analytical Methods, 9 (2017), 37; 5464 - 5471.

Modern chemical imaging techniques provide spatially resolved information on the molecular and
elemental composition of samples with both high spatial and spectral resolution. Over the past few
decades these techniques, in particular, Fourier transform infrared (FTIR) spectroscopy and laser ablation
inductively coupled plasma mass spectrometry (LA-ICP-MS) have been successfully applied in
histopathological research. This work demonstrates that the multivariate analysis of combined FTIR and
LA-ICP-MS microscopy hyperspectral images can bring additional knowledge to biomedical research.
The concept of such analysis was demonstrated while investigating two different tumor samples
subjected to anticancer therapy. Combined analysis has revealed a correlation between the lateral
distribution of analytes and sample properties within the different techniques. Correlations between
alterations in the average protein secondary structure and platinum distribution were found, as well as
between changes in the cell nuclear morphology and a reduction of physiologically relevant trace
elements. The results of combined analysis suggested different degrees of tumor viability. Univariate
analysis and k-means clustering successfully discriminated dead tumor regions and supported the results
of combined analysis.

http://publik.tuwien.ac.at/files/publik_266427.pdf