[Zurück]


Zeitschriftenartikel:

C. P. Goncalves, S. Milker, D. Kracher, M. Fink, F. Rudroff, R. Ludwig, A. Bommarius, M.D. Mihovilovic:
"More than 1000-fold stabilization of class B flavin monooxygenases in operandi without mutagenesis";
Advanced Synthesis & Catalysis, 359 (2017), S. 2121 - 2131.



Kurzfassung englisch:
This paper describesthe stabilizationof flavin-dependent monooxygenases under reaction conditions,using an engineered formulation of addi-tives (the natural cofactors NADPH andFAD,and superoxide dismutase and catalase as catalytic anti-oxidants). This way,a103-to104-foldincrease of the half-life was reached without resource-intensive di-rected evolution or structure-dependent protein en-gineering methods. Thestabilized enzymes are highly valuedfor theirsynthetic potential in biotech-nology and medicinalchemistry (enantioselective sulfur, nitrogen and Baeyer-Villiger oxidations;oxi-dative human metabolism), but widespread applica-tion was so far hindered by their notoriousfragility. Our technology immediately enables their use,does not require structural knowledge of the biocatalyst, and creates astrong basis for the targeted develop-ment of improvedvariants by mutagenesis.

Schlagworte:
biocatalysis;cofactors;enzyme stabiliza-tion;oxygenation;reactive oxygen species


"Offizielle" elektronische Version der Publikation (entsprechend ihrem Digital Object Identifier - DOI)
http://dx.doi.org/10.1002/adsc.201700585

Elektronische Version der Publikation:
http://publik.tuwien.ac.at/files/publik_266584.pdf


Erstellt aus der Publikationsdatenbank der Technischen Universitšt Wien.