Talks and Poster Presentations (with Proceedings-Entry):
F. Rudroff, C. P. Goncalves, D. Kracher, S. Milker, M. Fink, R. Ludwig, A. Bommarius, M.D. Mihovilovic:
"More Than 3000-Fold Stabilization Of Class B Flavin Monooxygenases In Operandi Without Time Consuming Protein Engineering";
Talk: Biotrans 2017,
Budapest, Ungarn (invited);
07-09-2017
- 07-13-2017; in: "Biotrans 2017, 13th International Symposium on Biocatalysis and Biotransformations",
(2017),
468 pages.
English abstract:
We developed a generic protocol for the stabilization of flavin-dependent monooxygenases under reaction conditions, using an engineered formulation of additives (natural cofactors and
catalytic antioxidants, Figure 1). This way, a 10^3-10^4-fold increase of the half-life was reached without resource-intensive directed evolution or structure-dependent protein engineering methods.
Furthermore, we validated our generic protocol for the stabilization of class B FMOs with three sequentially distant enzymes of this class.
Created from the Publication Database of the Vienna University of Technology.