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Talks and Poster Presentations (with Proceedings-Entry):

M. Lunzer, L. Shi, O.G. Andriotis, P. Gruber, M. Markovic, P.J. Thurner, D. Ossipov, R. Liska, A. Ovsianikov:
"Two-Photon Micropatterning of a Photolabile Hydrogel Using a Two-Photon Sensitizer";
Poster: Symposium 'BSTE 2018: Next Generation Tissue Engineering', Ghent, Belgien; 2018-11-21 - 2018-11-23; in: "BSTE 2018: Next Generation Tissue Engineering", (2018), PO18.



English abstract:
INTRODUCTION
Photolabile hydrogels are dynamically tunable smart materials for 3D cell encapsulation. Due to the integration of photocleavable moieties the cell surrounding matrix can be chemically or mechanically altered by the use of UV-VIS or pulsed NIR-laser light. In this contribution the effect of two-photon (2P) active small molecule P2CK1 on the 2P-cleavage of o-nitrobenzyl (oNB) junctions is discussed.
MATERIALS AND METHODS
A photolabile hydrogel was formed via thiol-ene Michael-addition by combining stock solutions of thiol modified hyaluronic acid (HA-SH, DS = 50%)2 and a photodegradable poly(ethylene glycol) (PEG) based cross-linker containing acrylate terminals.3 The hydrogel was then 2P-micropatterned at 800 nm in presence of cyclic benzylidene ketone P2CK1 and analyzed by AFM and LSM.
RESULTS AND DISCUSSION
By the use of P2CK as 2P-photosensitizer the threshold laser power for 2P-micropatterning of oNB-based hydrogels could be significantly decreased. This effect is demonstrated by laser scanning microscopy (LSM) and atomic force microscopy (AFM). Biocompatibility of both the promoted micropatterning process and the hydrogel platform is confirmed by cell culture studies involving adipose derived stem cells (ASCs).
CONCLUSIONS
Due to its modularity, the presented platform is a useful tool for fundamental studies of cell
matrix interactions in 3D where spatio-temporal control over the cell surrounding matrix by 2P-micropatterning of a photolabile hydrogel at moderate laser powers is needed.
Figure 1: GFP-labeled ASC/TERT1 encapsulated in PEG- HA-SH hydrogel. The LSM image was taken 7 d after 2P-mircopatterning in presence of 0.1 mM P2CK.
REFERENCES
[1] A. Ovsianikov, S. Mühleder, J. Torgersen, Z. Li, X.-H. Qin, S. Van Vlierberghe, P. Dubruel, W. Holnthoner, H. Redl, R. Liska, J. Stampfl, Langmuir 2014, 30, 3787-3794.
[2] A. M. Kloxin, M. W. Tibbitt, K. S. Anseth, Nat. Protoc. 2010, 5, 1867-1887.
[3] X. Z. Shu, Y. Liu, Y. Luo, M. C. Roberts, G. D. Prestwich, Biomacromolecules 2002, 3, 1304-1311.
ACKNOWLEDGEMENT
This work was supported by the Austrian Science Fund (FWF, Proj. Number I2444 N28).

Keywords:
dynamically tunable smart materials for 3D cell encapsulation, integration of photocleavable moieties, cell surrounding matrix can be chemically or mechanically altered by the use of UV-VIS or pulsed NIR-laser light

Created from the Publication Database of the Vienna University of Technology.