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D. Ret, G. Mihlaç, S. Gentilini, S. Knaus:
"Chemical characterization of hyaluronan derivatives (influence of proton mobility on accuracy of DS determination by NMR spectroscopy)";
Talk: 3rd International Conference on Biopolymers & Polymer Chemistry, 3rd International Conference on Biopolymers & Polymer Chemistry; Prague; 10-22-2018 - 10-23-2018; in: "3rd International Conference on Biopolymers & Polymer Chemistry", (2018), 3.

Hyaluronic acid (HA) is a biomaterial used in medical and esthetic application such as eye surgery, viscosupplementation therapy of osteoarthritis, dermal filling, and tissue augmentation. To improve the chemical and physical properties in biological systems hyaluronic acid is chemically modified. In establishment of the relationship between different degrees of modification and mechanical properties as well as biocompatibility the accurate determination of the degree of substitution (DS) is of great importance.
An effective and wide spread method for determining the DS is 1H-NMR spectroscopy. This method gives accurate results only when the implied protons are mobile. Hyaluronic acid proton mobility is affected by many parameters as: molecular weight, concentration, ionic force, pH and temperature.
In the present work the determination of the DS was intensively studied and the relationship between particle size, conformation in solution and proton mobility was elucidated. It will be shown that accurate determination of the DS by 1H-NMR spectroscopy for HA derivatives is possible and that the same principle can be used for other glycosaminoglycans.

Hyaluronic acid, chemically modified, chemical and physical properties in biological systems, the degree of substitution