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G. Ramer, F. S. Ruggeri, A. Centrone:
"Photothermal Induced Resonance Infrared Nanoscopy of Polypeptides in Water";
Poster: IRDG Christmas Meeting 2018, London, UK; 20.12.2018.

Photothermal induced resonance (PTIR) is a scanning probe technique that enables infrared spectroscopy and imaging at nanoscale lateral resolution. This capability enables the use of established infrared measurement procedures to new samples. For example, recently, PTIR has been used to determine the secondary structure of amyloid fibrils at a nanometer lateral scale. This work has however demonstrated an important restriction of PTIR, specifically that heretofore application in water has not been possible. Since the chemical environment of a protein strongly depends on its chemical environment, this means that the high lateral resolution is achieved at the cost of some uncertainty in the measurement result.
Here, we present a measurement scheme that enables PTIR in liquid and demonstrate that it can be used for protein secondary structure analysis.

AFM-IR, infrared, QCL, spectroscopy, nanoscale, optics, peptides, protein, secondary structure