[Zurück]

M. Marchetti-Deschmann, M. Holzlechner, A. Svirkova, M. Bonta, A. Turyanskaya, M. Großgarten, A. Balbekova, K. Wieland, B. Lendl, A. Limbeck:
"Correlative Multimodal Imaging in Mass Spectrometry";
Vortrag: 30th MassSpec-Forum Vienna, Vienna; 19.02.2019 - 20.02.2019; in: "Book of Abstracts", (2019), S. 24.

We present novel strategies to use MALDI Mass Spectrometry based imaging (MALDI-MSI) together with micro X-ray fluorescence (μ-XRF) or laser ablation inductive coupled plasma MS (LA-ICP-MS) for combined molecular and elemental imaging in biological tissues. MALDI-MS provides localized information on intact molecules, LA-ICP-MS gives quantitative elemental distribution information and μ-XRF imaging is successfully used to examine the spatial context of elements without destroying the sample.
We developed a sample preparation strategy allowing molecular and elemental analysis from the very same tissue section. We established a robust experimental workflow allowing for non-destructive elemental μXRF imaging to target representative elements for bone (Ca, P, S). The same tissue section was also measured by MALDI-MSI and lipid distributions in soft tissue like cartilage, blood vessels or the bone covering membrane (periosteum) could be accessed sensitively by MALDI-MSI. In a second attempt, MALDI-MSI was combined with LA-ICP-MS for quantitative elemental information. We aimed at lipid detection in positive and negative ion mode from the same tissue section while preserving elemental distributions for P, S and Pt targeted by LA-ICP-MS. We present MALDI-MSI data significantly correlating with high Pt concentrations in human malignant pleural mesothelioma samples after administration of individuals with platinum containing cytostatic drugs. Spatial segmentation of lipid data furthermore reflected determined Pt, P and S distributions and certain lipid species correlated with elemental concentrations.
Moreover, the successful combination of LA-ICP-MS, MALDI-MSI and FT-IR analysis is presented that allowed to better understand different inflammation events occurring in lung tissue after inhaling different types of SiO2 nanoparticles.

MALDI Mass Spectrometry, μ-XRF, LA-ICP-MS, FT-IR