S. Godehard, H. Müller, C. Badenhorst, C. Stanetty, C. Suster, M.D. Mihovilovic, U. Bornscheuer:
"Efficient Acylation of Sugars and Oligosaccharides in Aqueous Environment Using Engineered Acyltransferases";
ACS Catalysis, XXX (2021), 11; 5 S.

Kurzfassung englisch:
A major challenge for the enzymatic synthesis of sugar esters is the low
solubility of sugars in anhydrous, often toxic, organic solvents. We overcame this limitation
by using acyltransferases for efficient acetylation of sugars in water. Selective 6-Oacetylation of glucose, maltose, and maltotriose with conversions of up to 78% was achieved
within 15 min using engineered acyltransferases (4 μM). Moreover, we identified EstA as a
promiscuous acyltransferase preferentially acetylating sugars instead of hydrophobic acyl
acceptors. This expands the applicability of promiscuous acyltransferases to sugar
modifications and contributes to the understanding of how to adapt acyltransferases to
hydrophilic substrates.

acyl transfer, enzyme catalysis, promiscuous acyltransferase, protein engineering, sugar ester

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