S. Freitag, B. Baumgartner, S. Radel, A. Schwaighofer, A. Varriale, A. Pennacchio, S. DŽAuria, B. Lendl:
"A thermoelectrically stabilized aluminium acoustic trap combined with attenuated total reflection infrared spectroscopy for detection of Escherichia coli in water";
Lab on a Chip, 21 (2021), S. 1811 - 1819.

Kurzfassung englisch:
Acoustic trapping is a non-contact particle manipulation method that holds great potential for performingautomated assays. We demonstrate an aluminium acoustic trap in combination with attenuated totalreflection Fourier-transform infrared spectroscopy (ATR-FTIR) for detection ofE. coliin water. The thermalconductivity of aluminium was exploited to thermo-electrically heat and hold the acoustic trap at thedesired assay temperature of 37°C. Systematic characterisation and optimisation of the acoustic trapallowed high flow rates while maintaining high acoustic trapping performance. The ATR element serves notonly as a reflector for ultrasound standing wave generation but also as a sensing interface. The enzymeconversion induced by alkaline phosphatase-labelled bacteria was directly monitored in the acoustic trapusing ATR-FTIR spectroscopy. Sequential injection analysis allowed automated liquid handling, includingnon-contact bacteria retention, washing and enzyme-substrate exchange within the acoustic trap. Thepresented method was able to detectE. coliconcentrations as low as 1.95×106bacteria per mL in 197min. The demonstrated ultrasound assisted assay paves the way to fully automated bacteria detectiondevices based on acoustic trapping combined with ATR-FTIR spectroscop


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