Vorträge und Posterpräsentationen (mit Tagungsband-Eintrag):
P. Steinbauer, J. Kotmel, A. Rohatschek, O.G. Andriotis, R. Liska, P.J. Thurner, S. Baudis:
"Single-Molecule Force Spectroscopy Measurement of natural and biomimetic Adhesion Motifs";
Vortrag: ICBBA 2021 - 4th International Conference on Biological and Biomimetic Adhesives,
- 19.02.2021; in: "4th International Conference on Biological and Biomimetic Adhesives",
Achieving strong adhesion to substrates in water or physiological fluids remains as challenge in biomedical applications. To fully gain insight into biological adhesion processes and strategies for using them in technological applications, a fundamental expertise of different adhesion motifs and their binding mechanisms is crucial. Atomic force microscopy (AFM) in single-molecule force spectroscopy (SMFS) mode can be used to acquire detailed knowledge about individual molecules with adhesion motifs. Adhesion to a surface can be measured separately from cohesion. Phosphorylation of serine as well as the amino acid 3,4-dihydroxy-L-phenylalanine (DOPA) are of key importance in different natural adhesion processes. In this study, the adhesion of the specific phosphorylated amino acid sequence DpSpSEEKC, which was thought to be responsible for the strong binding ability in the protein statherin, was compared to the unphosphorylated sequence DSSEEKC via SMFS. Results showed that high adhesion forces over 1 nN on hydroxyapatite and on TiO2 are only present for the phosphorylated sequence. Based on these findings, two biomimetic phosphonate-containing block copolymers (28 kDa and 39 kDa) were synthesized, using reversible addition fragmentation-chain transfer (RAFT) polymerization. After tethering the polymers onto AFM tips, the adhesion was measured under physiological-like conditions on different substrates via SMFS. Results showed higher adhesion on hydroxyapatite, TiO2 and mica using polymers with longer phosphonate blocks. SMFS reveals new insights in natural adhesion mechanisms and emerges as promising tool for synthesizing new biomimetic polymers for tissue engineering applications.
physiological fluids, biomedical applications, biological adhesion processes, individual molecules with adhesion motifs, synthesizing new biomimetic polymers for tissue engineering applications
Erstellt aus der Publikationsdatenbank der Technischen Universitšt Wien.