[Zurück]

A. Schmidbauer, R. Reichsöllner, A. Puljic, J. Hackethal, H. Redl, S. Baudis:
"Neovascularization Stimulating Hydrogels for Regenerative Medicine";
Poster: TERMIS 6th World Congress, Maastricht; 15.11.2021 - 19.11.2021; in: "Book of Abstract", (2021), S. 1556.

Introduction
Neovascularization of implants used as scaffolds is crucial for the regeneration of large tissue defects. Hence, the formation
of vascular networks is of utmost importance. Photocrosslinkable hydrogels show promise as matrix for the ingrowth of
neo-vessels. We integrated the mimicry of placenta, a highly vascularized organ, within gelatin (Gel)-hydrogels. Different
Gel-modifications, including Gel-norbornene (Gel-NB) [1] and Gel-allyl glycidyl ether (Gel-AGE) [2] were in situ crosslinked
enabling fabrication of complex structures either by extrusion-based 3D-bioprinting or by two-photo-polymerization.[3]
Methods
Gel was modified with 5-norbornene-2,3-dicarboxylic anhydride,N-hydroxysuccinimide-activated 5-norbornene-2-carboxylic
acid (Gel-NBs), or allyl glycidyl ether (Gel-AGE) with degrees of modification. Hydrogel formation was monitored by
photorheology using lithium phenyl-2,4-6-trimethylbenzoylphosphinate, as photoinitiator and D,L-dithiothreitol or
ethoxylated trimethylolpropane tri(3-mercaptopropionate) as crosslinker in aqueous solution (2.5-10 wt%).[4].
Results
Mechanical properties (shear moduli 10-1000 Pa) and swellability (5-40 fold) of the hydrogels depend on the type/degree of
modification. Hydrogel vascular network formation with HUVECs was observed when placenta-specific factors were
incorporated.
Discussion and Conclusions
The formation of vascular networks could be observed with the prepared hydrogels. For the 3D fabrication of
vascularization promoting hydrogel constructs, suitable system is to be found by variation of parameter (modification,
crosslinker, gel content etc.).

Neovascularization Stimulating Hydrogels, Regenerative Medicine, formation of vascular networks, 3D fabrication