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Publications in Scientific Journals:

S. Balarastaghi, R. Birner-Grünberger, B. Bibi Marjan Razavi:
"Protective Effect of Crocin on Malathion-induced Cardiotoxicity in Rats: A Biochemical, Histopathological and Proteomics Study";
Iranian Journal of Pharmaceutical Research, 20 (2021), 111836.13385; 156 - 172.



English abstract:
In this study, the protective effect of crocin on malathion (MTN) induced cardiotoxicity
in rats in subacute exposure was evaluated. Rats were divided into 6 groups; control (normal
saline); MTN (100 mg/kg); MTN + crocin (10, 20 and 40 mg/kg) and MTN + vitamin E 200
IU/kg. Treatments were continued for two weeks. Creatine phosphokinase MB (CK-MB),
malondialdehyde (MDA) and glutathione (GSH) levels were evaluated in heart tissue at the end
of treatments. The effect of crocin and MTN on histopathological changes in rat cardiac tissue
was also investigated. The alteration of protein profile in the heart of the animals exposed to MTN
was evaluated by proteomic approach through two-dimensional gel electrophoresis followed by
matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) software. MTN induced
histopathological damages and elevated the level of cardiac marker CK-MB (P < 0.01). The level
of MDA increased and the level of GSH reduced (P < 0.001). MDA levels were reduced in all
crocin plus MTN groups (P < 0.001) and vitamin E plus MTN (P < 0.001) groups as compared to
MTN groups. However, in the crocin (10 mg/kg) + MTN group, the content of GSH compared to
MTN treated rats increased (P < 0.001). Protein abundance analysis identified proteins implicated
in cardiac necrosis, tricarboxylic acid cycle, cellular energy homeostasis, arrhythmias, heart
development, heart failure and cardiovascular homeostasis to be affected by MTN. In summary,
MTN may induce damage in the heart tissue of rats following subacute exposure and crocin, as
an antioxidant, showed protective effects against MTN cardiotoxicity.

Keywords:
Malathion; Crocin; Cardiotoxicity; Proteomics; Oxidative stress.


"Official" electronic version of the publication (accessed through its Digital Object Identifier - DOI)
http://dx.doi.org/10.22037/IJPR.2020.111836.13385

Electronic version of the publication:
https://publik.tuwien.ac.at/files/publik_301067.pdf


Created from the Publication Database of the Vienna University of Technology.