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S.M. Khoshfetrat, P. Hashemi, A. Afkhami, A. Hajian, H. Bagheri:
"Cascade electrochemiluminescence-based integrated graphitic carbon nitride-encapsulated metal-organic framework nanozyme for prostate-specific antigen biosensing";
Sensors and Actuators B: Chemical, 348 (2021), 130658; 1 - 10.

Developing sensitive strategies to detect the biomarkers is responsible for early detection of a genetic disorder and cancer-related biomarkers. Herein, a rational design of high catalytic activity of artificial enzyme mimics with enhancing the robustness of functional nanozymes has been introduced to detect PSA as a model target. Using a separation/enrichment tool of capture antibody (cAb1)-modified magnetic nanoparticles (MNPs/cAb1), signal amplifier luminol (LUM)-loaded graphitic carbon nitride nanosheets (C3N4 NS)-encapsulated NH2-MIL (53)-Fe (NMF) (C3N4@LUM/NMF)) to label signal antibody (Ab2) as a specific targeting and a supporting platform of a nanoporous gold electrode (NPGE) with high-efficiency electron acceleration, a cascade signal amplification strategy has been achieved. In addition, the nanoscale proximity effect of confined LUM within the C3N4@NMF nanozyme and synergistically enhanced catalytic activity causes significantly to promote the elec-trochemiluminescence (ECL) of LUM owing to minimize the decomposition of highly active intermediates of .OH-generating reaction of C3N4@NMF nanozymes from H2O2. Taking the advantages of multiple-amplification, the present strategy shows PSA detection in dynamic range of 0.1 pg mL

http://dx.doi.org/10.1016/j.snb.2021.130658