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U. Ramach:
"Comparison of protein interaction using membrane model systems and single-molecule measurements";
Vortrag: Seminar Institut für Allgemeine Physik (IAP), TU Wien, Institut für Angewandte Physik; 21.06.2022.

Protein-protein interactions regulate cell communication and are therefore important for our immune system. One protein of interest is the CD4 protein, which is a glycoprotein that serves as a co-receptor for the T-cell receptor (TCR) and can be found on different immune cells. Direct interaction between cells expressing CD4 and cells expressing its counterpart, the major histocompatibility complex class II (MHC II) is reported to be low due to its assisting role in the TCR-MHC II interaction. This interaction is happening in the vicinity of hundreds of different surface proteins as well as glycocalyx proteins like CD45, posing a challenge for accurately mimicking the protein interaction on model membranes.
This work gives an overview of choosing a model membrane system as well as protein-protein interaction using model membranes in SFA and single-molecule AFM.