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F. Reyzek, P. Bieber, T.M. Seifried, N. Bothen, R. Schwidetzky, U. Pöschl, M. Bonn, J. Fröhlich-Nowoisky, H. Grothe:
"Are proteinaceous agglomerates responsible for ice nucleation activity of birch pollen?";
Vortrag: EGU General Assembly 2022, Wien; 23.05.2022 - 27.05.2022; in: "EGU General Assembly 2022", EGU Copernicus, Wien (2022), S. #.

Various biological aerosol particles such as certain pollen, fungi, and bacteria are known as ice-nucleating particles with high onset freezing temperatures. It came as a surprise when Pummer et al. (2012) found that solubilized macromolecules were responsible for the ice nucleation activity of tree pollen, rather than the grains themselves. More recently, ice-nucleating macromolecules (INMs) have also been found on other tree tissues (Felgitsch et al., 2018, Seifried et al., 2020). In general, INMs are present in much greater numbers than the micrometer sized pollen grains and thus the emission of INMs from the biosphere might play a more important role than previously thought (Bieber et al., 2020, Burkart et al., 2021, Seifried et al., 2020, 2021).

So far, the chemical composition and structure of INMs remains largely unknown. To shine light on this, we extracted INMs from birch pollen with water and conducted various treatments, purification, and freezing experiments. For example, we detected ice nucleation activity after filtration through a 10 kDa cutoff filter. However, the concentration after 10 kDa filtration was comparatively lower than after 30 kDa or 50 kDa filtration suggesting that the INMs consist of agglomerates.

To concentrate the INMs we used ice affinity purification followed by treatment with acetone to precipitate proteins. We found high ice nucleation activity of this material, suggesting that the INM is an ice nucleating protein. Subsequently, size exclusion chromatography was used to isolate the INMs, leading us to a sample with high concentrations of INMs. Finally, the identification of INMs from trees will be the basis of understanding the mechanism of ice nucleation under atmospheric conditions.

bioareosols, pollen, ice nucleation, heterogeneous ice nucleation, ice nuclei, INM

http://dx.doi.org/10.5194/egusphere-egu22-12220